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生物降解 3

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农业微生物；产业发展；微生物肥料；饲用微生物；微生物农药；酶制剂微生物；微生物种业 1

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thermophilic anaerobic digestion of swine manure with sulfamethoxazole and norfloxacin: Dynamics of microbialcommunities and evolution of resistance genes

《环境科学与工程前沿（英文）》 2021年 第15卷 第5期 doi: 10.1007/s11783-020-1342-x

摘要：

• SMX addition had negative effect on acetoclastic methanogens in mesophilic AD.

关键词： Pig manure     Antibiotics     Anaerobic digestion     Resistance genes     Microbial community    

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Using pyrosequencing and quantitative PCR to analyze microbial communities

Husen ZHANG

《环境科学与工程前沿（英文）》 2011年 第5卷 第1期   页码 21-27 doi: 10.1007/s11783-011-0303-9

摘要： New high-throughput technologies continue to emerge for studying complex microbial communities. In particular, massively parallel pyrosequencing enables very high numbers of sequences, providing a more complete view of community structures and a more accurate inference of the functions than has been possible just a few years ago. In parallel, quantitative real-time polymerase chain reaction (QPCR) allows quantitative monitoring of specific community members over time, space, or different environmental conditions. In this review, the principles of these two methods and their complementary applications in studying microbial ecology in bioenvironmental systems are discussed. The parallel sequencing of amplicon libraries and using barcodes to differentiate multiple samples in a pyrosequencing run are explained. The best procedures and chemistries for QPCR amplifications are also described and advantages of applying automation to increase accuracy are addressed. Three examples in which pyrosequencing and QPCR were used together to define and quantify members of microbial communities are provided: in the human large intestine, in a methanogenic digester whose sludge was made more bioavailable by a high-voltage pretreatment, and on the biofilm anode of a microbial electrolytic cell. The key findings in these systems and how both methods were used in concert to achieve those findings are highlighted.

关键词： polymerase chain reaction (PCR)     microbial communities     pyrosequencing     gut     microbial fuel cell     sludge    

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INTERCROPPING TEA PLANTATIONS WITH SOYBEAN AND RAPESEED ENHANCES NITROGEN FIXATION THROUGH SHIFTS IN SOIL MICROBIALCOMMUNITIES

《农业科学与工程前沿（英文）》 2022年 第9卷 第3期   页码 344-355 doi: 10.15302/J-FASE-2022451

摘要：

● Intercropping change soil bacterial communities in tea plantations.

关键词： intercropping     rapeseed     soil microbe     soybean     tea garden    

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Removal of tetrachlorobisphenol A and the effects on bacterial communities in a hybrid sequencing biofilm

Xiaohui Wang, Shuai Du, Tao Ya, Zhiqiang Shen, Jing Dong, Xiaobiao Zhu

《环境科学与工程前沿（英文）》 2019年 第13卷 第1期 doi: 10.1007/s11783-019-1097-4

摘要：

SBBR-CW system was proposed to effectively treat wastewater containing TCBPA.

CW unit contributed more than SBBR to the removal of TCBPA.

TCBPA changed the composition and structure of bacterial community in the system.

GAOs massively grew in SBBR, but did not deteriorate TP removal efficiency.

关键词： SBBR     Constructed wetland     Tetrachlorobisphenol A     Microbial community structure    

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Nitrogen removal efficiencies and microbial communities in full-scale IFAS and MBBR municipal wastewater

Supaporn Phanwilai, Naluporn Kangwannarakul, Pongsak (Lek) Noophan, Tamao Kasahara, Akihiko Terada, Junko Munakata-Marr, Linda Ann Figueroa

《环境科学与工程前沿（英文）》 2020年 第14卷 第6期 doi: 10.1007/s11783-020-1374-2

摘要： Abstract • Two IFAS and two MBBR full-scale systems (high COD:N ratio 8:1) were characterized. • High specific surface area carriers grew and retained slow-growing nitrifiers. • High TN removal is related to high SRT and low DO concentration in anoxic tanks. The relative locations of AOB, NOB, and DNB were examined for three different kinds of carriers in two types of hybrid biofilm process configurations: integrated fixed-film activated sludge (IFAS) and moving bed biofilm reactor (MBBR) processes. IFAS water resource recovery facilities (WRRFs) used AnodkalnessTM K1 carriers (KC) at Broomfield, Colorado, USA and polypropylene resin carriers (RC) at Fukuoka, Japan, while MBBR WRRFs used KC carriers at South Adams County, Colorado, USA and sponge carriers (SC) at Saga, Japan. Influent COD to N ratios ranged from 8:1 to 15:1. The COD and BOD removal efficiencies were high (96%–98%); NH4+-N and TN removal efficiencies were more varied at 72%–98% and 64%–77%, respectively. The extent of TN removal was higher at high SRT, high COD:N ratio and low DO concentration in the anoxic tank. In IFAS, RC with high specific surface area (SSA) maintained higher AOB population than KC. Sponge carriers with high SSA maintained higher overall bacteria population than KC in MBBR systems. However, the DNB were not more abundant in high SSA carriers. The diversity of AOB, NOB, and DNB was fairly similar in different carriers. Nitrosomonas sp. dominated over Nitrosospira sp. while denitrifying bacteria included Rhodobacter sp., Sulfuritalea sp., Rubrivivax sp., Paracoccus sp., and Pseudomonas sp. The results from this work suggest that high SRT, high COD:N ratio, low DO concentration in anoxic tanks, and carriers with greater surface area may be recommended for high COD, BOD and TN removal in WRRFs with IFAS and MBBR systems.

关键词： IFAS     MBBR     AnodkalnessTM K1 carrier     Polypropylene resin carrier     Sponge carrier    

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Spatial and seasonal variations in bacterial communities of the Yellow Sea by T-RFLP analysis

Hongyuan WANG, Xiaolu JIANG, Ya HE, Huashi GUAN

《环境科学与工程前沿（英文）》 2009年 第3卷 第2期   页码 194-199 doi: 10.1007/s11783-009-0018-3

摘要： Four typical coastal sites (rocky shore, sandy shore, mud flat shore, and artificial harbor) at the Yellow Sea were chosen to investigate the spatial and seasonal variations in bacterial communities. This was accomplished by using terminal restriction fragment length polymorphism (T-RFLP) analysis of PCR amplified 16S rDNA fragments. Two kinds of tetrameric restriction enzymes, I and I, were used in the experiment to depict the bacterial community diversity in different marine environments. It was found that the community compositions digested by the two enzymes separately were different. However, the results of bacterial community diversity derived from them were similar. The MDA analysis results of T-RFLP profiles coming from I and I both exhibited a significant seasonal community shift for bacteria and a relatively low spatial variation among the four locations. With I as the sample, the pair wise -tests also revealed that variations were minor between each pair of marine environments, with ranging from 0.198 to 0.349. However, the bacterial community structure in the mud flat site depicted a larger difference than each of the other three sites ( ranging from 0.282 to 0.349).

关键词： terminal restriction fragment length polymorphism (T-RFLP)     bacterial community structure     marine microbial diversity     HhaI     MspI    

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functional gene microarrays in the analysis of the functional diversity, composition, and structure of microbialcommunities

Zhili HE, Joy D. VAN NOSTRAND, Ye DENG, Jizhong ZHOU

《环境科学与工程前沿（英文）》 2011年 第5卷 第1期   页码 1-20 doi: 10.1007/s11783-011-0301-y

摘要： Functional gene arrays (FGAs) are a special type of microarrays containing probes for key genes involved in microbial functional processes, such as biogeochemical cycling of carbon, nitrogen, sulfur, phosphorus, and metals, biodegradation of environmental contaminants, energy processing, and stress responses. GeoChips are considered as the most comprehensive FGAs. Experimentally established probe design criteria and a computational pipeline integrating sequence retrieval, probe design and verification, array construction, data analysis, and automatic update are used to develop the GeoChip technology. GeoChip has been systematically evaluated and demonstrated to be a powerful tool for rapid, specific, sensitive, and quantitative analysis of microbial communities in a high-throughput manner. Several generations of GeoChip have been developed and applied to investigate the functional diversity, composition, structure, function, and dynamics of a variety of microbial communities from different habitats, such as water, soil, marine, bioreactor, human microbiome, and extreme ecosystems. GeoChip is able to address fundamental questions related to global change, bioenergy, bioremediation, agricultural operation, land use, human health, environmental restoration, and ecological theories and to link the microbial community structure to environmental factors and ecosystem functioning.

关键词： functional gene arrays (FGAs)     GeoChip     microbial communities     functional diversity/composition/structure     environmental factor     ecosystem functioning    

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METAGENOMICS COMBINED WITH HIGH-THROUGHPUT SEQUENCING REVEALS THE METHANOGENIC POTENTIAL OF FRESH CORN STRAW UNDER THERMOPHILIC AND HIGH OLR

《农业科学与工程前沿（英文）》 2023年 第10卷 第3期   页码 403-423 doi: 10.15302/J-FASE-2022471

摘要：

● Methane production from fresh straw was 7.50% higher than dry straw.

关键词： fresh corn straw     high solid anaerobic digestion     metagenomics     microbial communities     thermophilic    

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Response of bacterial communities to short-term pyrene exposure in red soil

Jingjing PENG, Hong LI, Jianqiang SU, Qiufang ZHANG, Junpeng RUI, Chao CAI

《环境科学与工程前沿（英文）》 2013年 第7卷 第4期   页码 559-567 doi: 10.1007/s11783-013-0501-8

摘要： Pyrene, a representative polycyclic aromatic hydrocarbon (PAH) compound produced mainly from incomplete combustion of fossil fuels, is hazardous to ecosystem health. However, long-term exposure studies did not detect any significant effects of pyrene on soil microorganism. In this study, short-term microcosm experiments were conducted to identify the immediate effect of pyrene on soil bacterial communities. A freshly-collected pristine red soil was spiked with pyrene at 0, 10, 100, 200, and 500 mg·kg and incubated for one day and seven days. The bacterial communities in the incubated soils were analyzed using 16S rRNA sequencing and terminal restriction fragment length polymorphism (T-RFLP) methods. The results revealed high bacterial diversity in both unspiked and pyrene-spiked soils. Only at the highest pyrene-spiking rate of 500 mg·kg , two minor bacteria groups of the identified 14 most abundant bacteria groups were completely suppressed. Short-term exposure to pyrene resulted in dominance of Proteobacteria in soil, followed by Acidobacteria, Firmutes, and Bacteroidetes. Our findings showed that bacterial community structure did respond to the presence of pyrene but recovered rapidly from the perturbation. The intensity of impact and the rate of recovery showed some pyrene dosage-dependent trends. Our results revealed that different levels of pyrene may affect the bacterial community structure by suppressing or selecting certain groups of bacteria. It was also found that the bacterial community was most susceptible to pyrene within one day of the chemical addition.

关键词： pyrene     bacterial communities     terminal restriction fragment length polymorphism     short-term exposure     rank-abundance plots    

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Microbial communities biostimulated by ethanol during uranium (VI) bioremediation in contaminated sediment

Mary Beth LEIGH,Wei-Min WU,Erick CARDENAS,Ondrej UHLIK,Sue CARROLL,Terry GENTRY,Terence L. MARSH,Jizhong ZHOU,Philip JARDINE,Craig S. CRIDDLE,James M. TIEDJE

《环境科学与工程前沿（英文）》 2015年 第9卷 第3期   页码 453-464 doi: 10.1007/s11783-014-0721-6

摘要： Stable isotope probing (SIP) was used to identify microbes stimulated by ethanol addition in microcosms containing two sediments collected from the bioremediation test zone at the US Department of Energy Oak Ridge site, TN, USA. One sample was highly bioreduced with ethanol while another was less reduced. Microcosms with the respective sediments were amended with C labeled ethanol and incubated for 7 days for SIP. Ethanol was rapidly converted to acetate within 24 h accompanied with the reduction of nitrate and sulfate. The accumulation of acetate persisted beyond the 7 d period. Aqueous U did not decline in the microcosm with the reduced sediment due to desorption of U but continuously declined in the less reduced sample. Microbial growth and concomitant C-DNA production was detected when ethanol was exhausted and abundant acetate had accumulated in both microcosms. This coincided with U(VI) reduction in the less reduced sample. C originating from ethanol was ultimately utilized for growth, either directly or indirectly, by the dominant microbial community members within 7 days of incubation. The microbial community was comprised predominantly of known denitrifiers, sulfate-reducing bacteria and iron (III) reducing bacteria including , , , and others, including the known U(VI)-reducing bacteria A , and The findings suggest that ethanol biostimulates the U(VI)-reducing microbial community by first serving as an electron donor for nitrate, sulfate, iron (III) and U(VI) reduction, and acetate which then functions as electron donor for U(VI) reduction and carbon source for microbial growth.

关键词： Stable isotope probing (SIP)     ethanol     acetate     uranium reduction     sediment     bioremediation    

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氯霉素及其产物降解菌群在长期驯化过程中的微生物群落和关键基因演替模式

张家禹, 周开颜, 郭芳亮, 雷华新, 赵仁鑫, 林琳, 李晓岩, 李炳

《工程（英文）》 2023年 第31卷 第12期   页码 59-69 doi: 10.1016/j.eng.2023.07.009

摘要：

氯霉素（CAP）作为一种广泛存在的新污染物，对水环境中的生态群落产生了负面影响。生物处理广泛应用于水体污染物的去除，而功能微生物的性能决定了其效果。本研究从活性污泥中驯化了一个具有强大CAP降解能力的菌群。对硝基苯甲酸（PNB）和2,2-二氯乙酸（DCA）是CAP生物和化学处理过程中产生的主要产物，它们也分别作为菌群的唯一底物进行长期驯化。宏基因组分析揭示了2.5年驯化过程中微生物群落和关键功能基因的演替模式。经过长期驯化，Sphingomonas、Caballeronia和Cupriavidus分别成为CAP、PNB和DCA降解菌群中的优势菌属，并且它们分别是CAP、PNB和DCA的关键降解菌，它们之间的协同合作实现了CAP的高度矿化。PNB转化为原儿茶酸（PCA），然后通过间位和邻位裂解途径被矿化。通过长期驯化，参与CAP、PNB和DCA代谢的关键功能基因，包括CAP乙酰转移酶、CAP氧化还原酶、卤代酸脱卤酶和原儿茶酸双加氧酶，在菌群中显著富集。本研究还发现pH和碳源对CAP的生物降解效率有显著影响。本研究驯化的菌群和分离的菌株可用作加强CAP、PNB和DCA污染环境生物修复的微生物资源。

关键词： 抗生素     生物降解     代谢作用     微生物群落演替     宏基因组    

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Effects of riparian land use on water quality and fish communities in the headwater stream of the Taizi

Sen DING, Yuan ZHANG, Bin LIU, Weijing KONG, Wei MENG

《环境科学与工程前沿（英文）》 2013年 第7卷 第5期   页码 699-708 doi: 10.1007/s11783-013-0528-x

摘要： Riparian land use remains one of the most significant impacts on stream ecosystems. This study focuses on the relationship between stream ecosystems and riparian land use in headwater regions. Four riparian land types including forest, grassland, farmland, and residential land were examined to reveal the correlation between stream water and fish communities in headwater streams of the Taizi River in north-eastern China. Four land types along riparian of 3 km in length were evaluated at 25, 50, 100, 200 and 500 m widths, respectively. Generally, the results found a significant relationship between riparian land uses and stream water quality. Grassland was positively correlated with water quality parameters (conductivity and total dissolved solids) at scales from 100 to 500 m riparian width. Farmland and residential land was negatively correlated with water quality parameters at scales from 25 to 500 m and from 50 to 200 m riparian widths, respectively. Although the riparian forest is important for maintaining habitat diversity and fish communities, the results found that only fish communities were significantly correlated with the proportion of riparian farmland. Farmland had a positive correlation with individual fish abundance within a riparian corridor of 25 to 50 m, but a negative correlation with fish diversity metrics from 25 to 100 m. This study indicates that effective riparian management can improve water quality and fish communities in headwater streams.

关键词： fish     water quality     land use     riparian buffer     headwater stream    

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Characterization of bacterial communities during persistent fog and haze events in the Qingdao coastal

《环境科学与工程前沿（英文）》 2021年 第15卷 第3期 doi: 10.1007/s11783-020-1334-x

摘要：

• Light haze had little effect on bacterial communities.

关键词： Bacterial community     Persistent fog and haze     Particle size    

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Responses of microbial interactions to elevated salinity in activated sludge microbial community

《环境科学与工程前沿（英文）》 2023年 第17卷 第5期 doi: 10.1007/s11783-023-1660-x

摘要：

● Salinity led to the elevation of NAR over 99.72%.

关键词： Elevated salinity     Activated sludge system     Pollution removal     Microbial interactions     Competitive relationship    

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Spectral characteristics of plant communities from salt marshes: A case study from Chongming Dongtan,

ZHANG Liquan, GAO Zhanguo, Armitage Richard, Kent Martin

《环境科学与工程前沿（英文）》 2008年 第2卷 第2期   页码 187-197 doi: 10.1007/s11783-008-0004-1

摘要： The spectral reflectance of recently formed salt marshes at the mouth of the Yangtze River, which are undergoing invasion by , were assessed to determine the potential utility of remotely sensed data in assessing future invasion and changes in species composition. Following a review of published research on remote sensing of salt marshes, 53 locations along three transects were sampled for paired data on plant species composition and spectral reflectance using a FieldSpec Pro JR Field Portable Spectroradiometer. Spectral data were processed concerning reflectance, and the averaged reflectance values for each sample were re-analysed to correspond to a 12-waveband bandset of the Compact Airborne Spectral Imager. The spectral data were summarised using principal components analysis (PCA) and the relationships between the vegetation composition, and the PCA axes of spectral data were examined. The first PCA axis of the reflectance data showed a strong correlation with variability in near infrared reflectance and ‘brightness’, while the second axis was correlated with visible reflectance and ‘greenness’. Total vegetation cover, vegetation height, and mudflat cover were all significantly related to the first axis. The implications of this in terms of the ability of remote sensing to distinguish the various salt marsh species and in particular the invasive species were discussed. Major differences in species with various physiognomies could be recognised but problems occurred in separating early colonising from other species at that stage. Further work using multi-seasonal hyperspectral data might assist in solving these problems.

关键词： Spectral     multi-seasonal hyperspectral     invasive     JR     re-analysed    

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标题 作者 时间 类型 操作

thermophilic anaerobic digestion of swine manure with sulfamethoxazole and norfloxacin: Dynamics of microbialcommunities and evolution of resistance genes

期刊论文

Using pyrosequencing and quantitative PCR to analyze microbial communities

Husen ZHANG

期刊论文

INTERCROPPING TEA PLANTATIONS WITH SOYBEAN AND RAPESEED ENHANCES NITROGEN FIXATION THROUGH SHIFTS IN SOIL MICROBIALCOMMUNITIES

期刊论文

Removal of tetrachlorobisphenol A and the effects on bacterial communities in a hybrid sequencing biofilm

Xiaohui Wang, Shuai Du, Tao Ya, Zhiqiang Shen, Jing Dong, Xiaobiao Zhu

期刊论文

Nitrogen removal efficiencies and microbial communities in full-scale IFAS and MBBR municipal wastewater

Supaporn Phanwilai, Naluporn Kangwannarakul, Pongsak (Lek) Noophan, Tamao Kasahara, Akihiko Terada, Junko Munakata-Marr, Linda Ann Figueroa

期刊论文

Spatial and seasonal variations in bacterial communities of the Yellow Sea by T-RFLP analysis

Hongyuan WANG, Xiaolu JIANG, Ya HE, Huashi GUAN

期刊论文

functional gene microarrays in the analysis of the functional diversity, composition, and structure of microbialcommunities

Zhili HE, Joy D. VAN NOSTRAND, Ye DENG, Jizhong ZHOU

期刊论文

METAGENOMICS COMBINED WITH HIGH-THROUGHPUT SEQUENCING REVEALS THE METHANOGENIC POTENTIAL OF FRESH CORN STRAW UNDER THERMOPHILIC AND HIGH OLR

期刊论文

Response of bacterial communities to short-term pyrene exposure in red soil

Jingjing PENG, Hong LI, Jianqiang SU, Qiufang ZHANG, Junpeng RUI, Chao CAI

期刊论文

Microbial communities biostimulated by ethanol during uranium (VI) bioremediation in contaminated sediment

Mary Beth LEIGH,Wei-Min WU,Erick CARDENAS,Ondrej UHLIK,Sue CARROLL,Terry GENTRY,Terence L. MARSH,Jizhong ZHOU,Philip JARDINE,Craig S. CRIDDLE,James M. TIEDJE

期刊论文

氯霉素及其产物降解菌群在长期驯化过程中的微生物群落和关键基因演替模式

张家禹, 周开颜, 郭芳亮, 雷华新, 赵仁鑫, 林琳, 李晓岩, 李炳

期刊论文

Effects of riparian land use on water quality and fish communities in the headwater stream of the Taizi

Sen DING, Yuan ZHANG, Bin LIU, Weijing KONG, Wei MENG

期刊论文

Characterization of bacterial communities during persistent fog and haze events in the Qingdao coastal

期刊论文

Responses of microbial interactions to elevated salinity in activated sludge microbial community

期刊论文

Spectral characteristics of plant communities from salt marshes: A case study from Chongming Dongtan,

ZHANG Liquan, GAO Zhanguo, Armitage Richard, Kent Martin

期刊论文